Human C-Reactive Protein (hCRP) Pincer^TM Assay

Catalog No: CH1008

This hCRP assay kit is to be used for the in vitro quantitative determination of hCRP concentrations in solution, serum, plasma and urine.

Human C-Reactive Protein (hCRP) is a calcium-dependent ligand-binding plasma protein¹, which is composed of five identical nonglycosylated polypeptide subunits (M.W. 23,027), each containing 206 amino acid residues. The monomers are noncovalently associated in an annular configuration with cyclic pentameric symmetry². hCRP is mainly produced by adipocytes³ and the hCRP concentration is a very useful nonspecific biochemical marker of inflammation. In healthy young adult volunteer blood donors, the median concentration of hCRP is 0.8 mg/L, however, following an acute-phase stimulus, values may increase to more than 500 mg/L⁴. High plasma concentration of hCRP is also associated with increased risk of stroke, myocardial infarction, and other vascular disease^5,6.

This hCRP assay is a rapid, homogeneous antibody-based fluorescence assay for detecting hCRP in biological samples. This assay consist two hCRP specific antibodies which recognize different epitopes on hCRP protein. Each antibody is modified with short complementary single-stranded DNAs which associate to form a duplex in the presence of the hCRP. FRET (Fluorescence Resonance Energy Transfer) is initiated between the two fluorophores added to the end of the two DNAs.  The amount of FRET signal is associated with the hCRP protein concentration presented in the samples⁷. This assay is simple (one step) and fast (30 min).

hCRP Pincer^TM Assay - Standard Curves

The following hCRP standard curve was prepared using the hCRP Pincer^TM Assay kit. This standard curve is for the purpose of illustration only, and should not be used to calculate unknowns. Each user should obtain his own data and standard curve.

hCRP Pincer^TM Assay – Sensitivity And Specificity

Sensitivity: The minimum detectable concentration of hCRP by this assay is estimated to be 5 ng/ml.

Specificity: The hCRP Pincer^TM Assay Kit is specific to hCRP and will not cross react with various related antigens.

Reference:

1. Pepys MB, Hirschfield GM (2003). C-reactive protein: a critical update. J. Clin. Invest. 111 (12): 1805–12.
2. Thompson, D, Pepys, MB, Wood, SP (1999). The physiological structure of human C-reactive protein and its complex with phosphocholine. Structure. 7 (2): 169-177.
3. Lau DC, Dhillon B, Yan H, Szmitko PE, Verma S (2005). Adipokines: molecular links between obesity and atheroslcerosis. Am. J. Physiol. Heart Circ. Physiol. 288 (5): H2031–2041.
4. Shine, B, de Beer, FC, Pepys, MB (1981). Solid phase radioimmunoassays for C-reactive protein. Clin. Chim. Acta. 117:13-23.
5. Ridker PM, Glynn RJ, Hennekens CH (1998). C-reactive protein adds to the predictive value of total and HDL cholesterol in determining risk of first myocardial infarction. Circulation. 97:2007–2011.
6. Lloyd-Jones DM, Liu K, Tian L, Greenland P (2006). Narrative Review: Assessment of C-Reactive Protein in Risk Prediction for Cardiovascular Disease. Ann Intern Med 145 (1): 35–42.
7. Heyduk, T., & Heyduk, E. (2005). Nucleic acid-based fluorescence sensors for detecting proteins. Anal. Chem., 77:1147-1156.

RESEARCH USE:

For research use only, not for use in diagnostic procedures.

Patent pending.

Product name	Measurements	Cat. #	Protocol	MSDS	Price	Order
hCRP PincerTMAssay kit	100	CH1008-100			$200
hCRP PincerTMAssay kit	384	CH1008-384			$420
384-well round-bottom low volume non-binding surface black polystyrene microplate	1 plate	163301	--		$9.50