Mouse IgG Pincer^TM Assay

Catalog No: MIG1012

In mice, IgG comprises four subclasses : IgG1, IgG2a, IgG2b and IgG3. Mouse IgG1 plays an important role in recognition of carbohydrate epitopes. IgG2a and IgG2b have the ability to fix Complement and recognize protein antigens. IgG3 is mainly involved in binding to Mast cells [Hussain R et al. (1995) Clin Diagn Lab Immunol. 2(6): 726-732] Individual subclasses vary with respect to their initial amino acid sequence, their physical and chemical properties.

This Mouse IgG pincer^TM Assay is a rapid, homogeneous antibody-based fluorescence assay for detecting and quantifying mouse IgG in biological samples such as ascitic fluids and hybridoma cell cultures. The assay uses two mouse IgG pincers that are anti-mouse IgG specific antibody conjugated to short complementary single-stranded oligonucleotides. In the presence of mouse IgG, the two single-stranded oligos associate to form a duplex and FRET (Fluorescence resonance energy transfer) is initiated between the two fluorophores labelled at the end of the two oligonucleotides. The amount of FRET signal is associated with the IgG concentration present in the samples. This assay is simple (one step) and fast (30 min).

Mouse IgG Pincer^TM Assay - Standard Curves

The following mouse IgG standard curve was prepared using the Mouse IgG Pincer^TM Assay kit. This standard curve is for the purpose of illustration only, and should not be used to calculate unknowns. Each user should obtain his own data and standard curve.

Mouse IgG Pincer^TM Assay – Sensitivity And Specificity

Sensitivity: The minimum detectable concentration of mouse IgG by this assay is estimated to be 30ng/mL.

Detection range: 30- 1000ng/mL.

RESEARCH USE:

For research use only, not for use in diagnostic procedures.

Patent pending.

Product name	Measurements	Cat. #	Protocol	MSDS	Price	Order
Mouse IgG PincerTMAssay kit	100	MIG1012-100			$180
Mouse IgG PincerTMAssay kit	384	MIG1012-384			$320
Mouse IgG PincerTMAssay kit	384 x 2	MIG1012-384 x 2			$580
Mouse IgG PincerTMAssay kit	384 x 3	MIG1012-384 x 3			$800
384-well round-bottom low volume non-binding surface black polystyrene microplate	1 plate	163301	--		$9.50