Rabbit IgG Pincer^TM Assay

Catalog No: RI1015

This Rabbit IgG pincer^TM Assay is a rapid, homogeneous antibody-based fluorescence assay for detecting and quantifying rabbit IgG in cell culture supernatants or other biological fluids.

The assay uses two rabbit IgG pincers that are anti-rabbit IgG specific antibody conjugated to short complementary single-stranded oligonucleotides. In the presence of rabbit IgG, the two single-stranded oligos associate to form a duplex and FRET (Fluorescence resonance energy transfer) is initiated between the two fluorophores labelled at the end of the two oligonucleotides. The amount of FRET signal is associated with the IgG concentration present in the samples. This assay is simple (one step) and fast (30 min).

Rabbit IgG Pincer^TM Assay - Standard Curves

The following rabbit IgG standard curve was prepared using the Rabbit IgG Pincer^TM Assay kit. This standard curve is for the purpose of illustration only, and should not be used to calculate unknowns. Each user should obtain his own data and standard curve.

Rabbit IgG Pincer^TM Assay – Sensitivity And Specificity

Sensitivity: The minimum detectable concentration of rabbit IgG by this assay is estimated to be 30ng/mL.

Detection range: 30- 1000ng/mL.

RESEARCH USE:

For research use only, not for use in diagnostic procedures.

Patent pending.

For 96-well format please inquire. Click Here.

Product name	Measurements	Cat. #	Protocol	MSDS	Price	Order
Rabbit IgG PincerTMAssay kit	100	RI1015-100			$180
Rabbit IgG PincerTMAssay kit	384	RI1015-384			$320
Rabbit IgG PincerTMAssay kit	384 x 2	RI1015-384 x 2			$580
Rabbit IgG PincerTMAssay kit	384 x 3	RI1015-384 x 3			$800
384-well round-bottom low volume non-binding surface black polystyrene microplate	1 plate	163301	--		$9.50