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Cells were incubated with isobutyl-1-methylxanthine (0.5 mM) before the addition of Cry1Ab, and cAMP was measured by using the Bridge-It cAMP Assay (Mediomics) in S5 and H5 cells (3 x 104).” 22. White CA, Sowa JR, Blake AD. A fluorescence-based second messenger assay to identify small molecule antagonists of somatostatin. 2006, ASPET, 48 (1). -- " Intracellular cAMP levels were quantified using a Bridge-It cAMP Designer Fluorescence Assay (Mediomics, LLC, St Louis, MO), which replaced more conventional radioimmunoassay methods." 23. Keys JR, Zhou RH, Harris DM, Druckman CA, Eckhart AD. (2005) Vascular smooth muscle overexpression of G protein-coupled receptor kinase 5 elevates blood pressure, which segregates with sex and is dependent on Gi-mediated signaling. Circulation. 112:1145-1153. --“cAMP accumulation was measured by use of a Bridge-It cAMP assay kit according to the manufacturer’s instructions (Mediomics, LLC).” 24. Calebiro D, de Filippis T, Lucchi S, Covino C, Panigone S, Beck-Peccoz P, Dunlap D, Persani L. (2005) Intracellular entrapment of wild-type TSH receptor by oligomerization with mutants linked to dominant TSH resistance. Hum Mol Genet. 14:2991-3002. 25. Bernier SG, Lazarus DD, Clark E, Doyle B, Labenski MT, Thompson CD, Westlin WF, Hannig G. (2004) A methionine aminopeptidase-2 inhibitor, PPI-2458, for the treatment of rheumatoid arthritis. Proc Natl Acad Sci U S A. 101:10768-10773. --“For protein detection in cell lysates, we used MetAP-2 polyclonal antibody CM33 (Zymed), proliferating cell nuclear antigen (PCNA) monoclonal antibody PC-10 (Santa Cruz Biotechnology), and MetAP-1 polyclonal antibody (Mediomics, St. Louis).” 26. Cooper AC, Karp RM, Clark EJ, Taghizadeh NR, Hoyt JG, Labenski MT, Murray MJ, Hannig G, Westlin WF, Thompson CD. (2006) A novel methionine aminopeptidase-2 inhibitor, PPI-2458, inhibits non-Hodgkin's lymphoma cell proliferation in vitro and in vivo. 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Products in the Press 1. Feature Article: “Tools And Technologies For Signal Transduction Studies” Technology Spotlight. Biocompare. Jul 21 '03. http://www.biocompare.com/spotlight.asp?id=146 2. Feature Article: William J Thomsen, Joel Gatlin, David J Unett, and Dominic P Behan. “Developing Functional GPCR Screens.” Current Drug Discovery. Jan. 2004 |
